The phasor plots for FLIM and Hyperspectral imaging: fundamentals and its applications. L. Malacrida

The phasor plot approach is a model-free method to analyze and interpret time-resolved (FLIM) and hyperspectral imaging (HSI). Traditionally, FLIM or HSI analysis requires a data fitting or a demixing model. The model selection requires specific knowledge, which restricts its adoption of the need for expertise to succeed in the data analysis and interpretation. Due to the model-free fashion, intuitiveness, and simple rules, the phasor plots are democratizing the use of these advanced imaging technologies. In this seminar, I will cover the fundamentals and their applications for addressing different biophysical problems straightforward. For instance, the role of water dynamics and molecular crowding in cells and animals will be elucidated by the phasor approach in combination with dimethylamino-naphthalene probes or the harmonic content for the hyperspectral unmixing of many components. Finally, I will share the technological developments at the Advanced Bioimaging Unit (UBA), and the many opportunities for students and researchers can visit and run research at the UBA.

Leonel Malacrida, PhD. (Advanced Bioimaging Unit - IPMon-Udelar) journey to advanced bioimaging started during his PhD research in biophysics in Uruguay when he first studied lung surfactant -a biofilm coating the lung interior that enables us to breathe- and wanted to understand this biological material at the molecular level. During his postdoc at the Laboratory for Fluorescence Dynamics at the University of California, Irvine, he delved into opportunities using advanced microscopy to quantitatively evaluate biology. Leonel is particularly interested in developing methods to image cells hidden in deep layers of tissues not accessible to normal microscopes. As an Associate Professor in the School of Medicine at the Universidad de la República of Uruguay, he recently established the Advanced Bioimaging Unit, a world-class facility for the development of novel instrumentation and for providing services and training to researchers throughout Uruguay. He is deeply committed to training the next generation of young researchers in modern advanced microscopy.

Last modified: Monday, 15 August 2022, 9:22 PM